Integrated & Automated

Single Instrument, Walkaway Operation

Every component of the Rebus EsperTM has been developed to work together to deliver high-resolution, high-throughput data while maintaining ease of use and walkaway operation.


Read more about the Rebus Esper

Synthetic Aperture Optics (SAO)

At the heart of the Rebus Esper is our patented Synthetic Aperture Optics or SAO, which allows image-based spatial analysis of single cells with no compromise between resolution, scale and speed.

In SAO, the sample is illuminated by a series of high-resolution light patterns that are created by the interference of excitation laser beams.

A series of low-resolution images are captured by a 20X air objective and automatically reconstructed using proprietary algorithms to generate a single high-resolution image.

The reconstructed image has the equivalent resolution and sensitivity of an image captured with a high numerical aperture 100X oil immersion lens.

The result: rapid analysis of full tissue sections with pristine subcellular detail.

On-system Chemistry

On top of our advanced optics engine, we’ve built an on-system fluidics system that handles all sample treatments automatically.

The system was designed with flexibility in mind, to enable multiple Rebus Biosystems-developed assays of multiple analytes.

All assays are validated and optimized for the Rebus Esper and paired with control software that maximizes ease of use.

Our purpose-built fluidics system and flow cell deliver reagents using the optimal combination of flow rate and temperature.

On-board refrigeration keeps solutions at the right temperature for the duration of experiments.

Precise, high-speed temperature control within the flow cell allows for the fastest possible reaction times, minimizing the time it takes for you to go from sample to data.

Intuitive Software

The EsperTM Spatial Studio is our software suite that takes the high-resolution imaging data acquired by the Rebus Esper and processes it into formats you can use directly for single-cell analysis and spatial mapping.

As images are acquired with SAO, they’re processed on the fly so that a final, analysis-ready data package is ready soon after your run ends.

The Esper Process software uses state-of-the-art computer vision algorithms to detect features, such as RNA spots, segment nuclei based on DAPI, and assign features to nuclei.

The tissue-wide CellxFeature matrix is the final output of Esper Process. It is a single file containing single cell data for hundreds of thousands of cells and millions of cellular features…all spatially annotated and ready for downstream analysis.

Esper Explore, our visualization software based on the open-source project Napari, allows convenient visualization, exploration and editing of all of Esper’s multi-dimensional data.