Cerebrovascular diseases are a leading cause of death and neurologic disability. Further understanding of disease mechanisms and therapeutic strategies requires a deeper knowledge of cerebrovascular cells in humans. We profiled transcriptomes of 181,388 cells to define a cell atlas of the adult human cerebrovasculature, including endothelial cell molecular signatures with arteriovenous segmentation and expanded perivascular cell diversity.
The human brain is subdivided into distinct anatomical structures, including the neocortex, which in turn encompasses dozens of distinct specialized cortical areas. Early morphogenetic gradients are known to establish early brain regions and cortical areas, but how early patterns result in finer and more discrete spatial differences remains poorly understood…
Batch effects due to technical variability are a major problem in single cell transcriptomics. Spatial methods are no exception – their low throughput requires high numbers of technical replicates, reducing the statistical power needed to quantify differential gene expression across experimental conditions…
Using an automated, multiplexed single-molecule fluorescent in situ hybridization (smFISH) approach, we validated the expression pattern of area-specific neuronal genes and also discover that laminar gene expression patterns are highly dynamic across cortical regions…
To dissect cell-type composition and visualize cell clusters in space, we designed a panel of genes identified by dissociated single-cell transcriptomics according to their specificity and expression. Multiple regions of fresh frozen human fetal brain samples from gestational week 20 were sectioned to a glass coverslip…
Here, we used the Rebus Biosystems platform to quantify expression levels of 31 target genes across three experimental conditions. First, we obtained fresh frozen mouse brain sections from two transgenic lines modeling a neurodegenerative disease and a third wild-type line serving as a control…
Various scRNA-seq approaches enable comprehensive screening of complete tissues but require tissue dissociation and the removal of individual cells from their biological context. Validation of differential gene expression in situ is, therefore, of high interest…
Single-cell RNA sequencing (scRNA-seq) has fundamentally expanded our understanding of tissue composition and heterogeneity. While this methodology enables unbiased and comprehensive identification of distinct cell types based on their transcriptomic profiles, it does not provide positional context in the tissue architecture, which is critical for understanding the interactions between cells and their native tissue microenvironment…
Cooperation between DNA, RNA and protein regulates gene expression and controls differentiation through interactions connecting regions of nucleic acids and protein domains and through the assembly of biomolecular condensates…
Mutations in the promoter region of the gene coding for the reverse transcriptase component of telomerase (TERT) are found at high frequency in various human tumor types…
Long noncoding RNAs (lncRNAs) exhibit diverse functions, including regulation of development. Here the authors combined genome-wide mapping of SMAD3 occupancy with expression analysis to identify lncRNAs induced by activin signaling during endoderm differentiation of human embryonic stem cells (hESCs)…
In conventional light microscopy, the physics of lenses impose relations between resolution and other optical parameters of the system. For example, the numerical aperture of a lens cannot be increased to improve resolution without either increasing the size of the lens or decreasing the working distance…
