The Rebus Esper brings together advancements in imaging, fluidics, chemistry and bioinformatics to deliver spatial omics data without compromise. Rebus Biosystems Senior Product Manager Brett Cook explains more about how this revolutionary system brings quantitative single-molecule, single-cell spatial data with subcellular resolution to your research.
In conventional light microscopy, the physics of lenses impose relations between resolution and other optical parameters of the system. For example, the numerical aperture of a lens cannot be increased to improve resolution without either increasing the size of the lens or decreasing the working distance. The technique presented here, Synthetic Aperture Optics (SAO), removes […]
Long noncoding RNAs (lncRNAs) exhibit diverse functions, including regulation of development. Here the authors combined genome-wide mapping of SMAD3 occupancy with expression analysis to identify lncRNAs induced by activin signaling during endoderm differentiation of human embryonic stem cells (hESCs). They found that DIGIT is divergent to Goosecoid (GSC) and expressed during endoderm differentiation. Using single […]
Cooperation between DNA, RNA and protein regulates gene expression and controls differentiation through interactions connecting regions of nucleic acids and protein domains and through the assembly of biomolecular condensates. In this study, the authors used StellarVision, a Synthetic Aperture Optics-based imaging system, to measure interactions between DIGIT, a long non-oding RNA, and BRD3, a protein […]
Mutations in the promoter region of the gene coding for the reverse transcriptase component of telomerase (TERT) are found at high frequency in various human tumor types. These TERT promoter mutations (TPMs) have been detected both in early- and late-stage tumors, consistent with the possibility that they play a role early in tumorigenesis. SAO-based imaging […]
Single-cell RNA sequencing (scRNA-seq) has fundamentally expanded our understanding of tissue composition and heterogeneity. While this methodology enables unbiased and comprehensive identification of distinct cell types based on their transcriptomic profiles, it does not provide positional context in the tissue architecture, which is critical for understanding the interactions between cells and their native tissue microenvironment. […]
Various scRNA-seq approaches enable comprehensive screening of complete tissues but require tissue dissociation and the removal of individual cells from their biological context. Validation of differential gene expression in situ is, therefore, of high interest. Rebus Biosystems has developed an automated platform that combines Synthetic Aperture Optics, fluidics engineering, and single-molecule RNA fluorescence in-situ hybridization […]
Here, we used the Rebus Biosystems platform to quantify expression levels of 31 target genes across three experimental conditions. First, we obtained fresh frozen mouse brain sections from two transgenic lines modeling a neurodegenerative disease and a third wild-type line serving as a control. We mounted one section from each of the three conditions (genotypes) […]
To dissect cell-type composition and visualize cell clusters in space, we designed a panel of genes identified by dissociated single-cell transcriptomics according to their specificity and expression. Multiple regions of fresh frozen human fetal brain samples from gestational week 20 were sectioned to a glass coverslip, assembled into the platform’s hardware, and automatically probed for […]
Using an automated, multiplexed single-molecule fluorescent in situ hybridization (smFISH) approach, we validated the expression pattern of area-specific neuronal genes and also discover that laminar gene expression patterns are highly dynamic across cortical regions. Together, our data suggest that early cortical areal patterning is defined by strong, mutually exclusive frontal and occipital gene expression signatures, with resulting […]
SANTA CLARA, Calif. – Rebus Biosystems, Inc., a venture-backed Silicon Valley-based life science technology company creating revolutionary tools to enable spatial omics research without compromise, today announced the appointment of Neil Kennedy as Chief Commercial Officer (CCO) and Scott Kothlow as Chief Financial Officer (CFO). This news comes on the heels of the company’s recent launch of the Rebus EsperTM spatial omics platform […]
