SANTA CLARA, CA – March 17, 2022 – Rebus Biosystems Inc, (“Rebus Bio”, “Rebus”), an Illumina Ventures-backed life science technology company creating revolutionary tools to advance spatial omics research, announced today that Arnold Kriegstein, M.D., PhD. and Kun Zhang, PhD., have been appointed to the Company’s Scientific Advisory Board (SAB). Both Drs. Kriegstein and Zhang […]
Mapping the Elusive: Analysis Of Cellular Expression Using the Esper as a Method for Detailed Tissue Mapping and Structural Patterns Recognition
Brett Cook, PhD, Senior Product Manager at Rebus provides an overview of the Esper system. Marcos Otero-Garcia, PhD, Senior Scientist at Rebus, compares mouse disease models of neurodegeneration with the Esper. (11:52) Simone Codeluppi, PhD, Principal Scientist of Bioinformatics, discusses spatial organization of cell types in the mouse cortex revealed by multiplexed single-molecule fluorescent in […]
SANTA CLARA, CA – Nov. 02, 2021 – Rebus Biosystems Inc, (“Rebus Bio”, “Rebus”), an Illumina Ventures-backed life science technology company creating revolutionary tools to advance spatial omics research, today announced that Drs. Arul Chinnaiyan, MD, PhD and Sten Linnarsson, PhD, have been appointed to the Company’s Scientific Advisory Board (SAB). The two globally recognized scientific advisers bring a […]
San Francisco – Sept. 20, 2021 – Illumina Ventures, an independently-managed, healthcare-focused venture firm, announced today the closing of its second investment fund with total commitments of $325 million, bringing its total capital under management to $560 million. The latest fund is anchored by Illumina, Inc. (Nasdaq: ILMN), with the majority of the total committed capital […]
The human brain is subdivided into distinct anatomical structures, including the neocortex, which in turn encompasses dozens of distinct specialized cortical areas. Early morphogenetic gradients are known to establish early brain regions and cortical areas, but how early patterns result in finer and more discrete spatial differences remains poorly understood. Here we use single-cell RNA […]
Dr. Marcos Otero-Garcia, Sr. Scientist.
Dr. Simone Codeluppi, Principle Scientist.
Dr. Brett Cook, Sr. Product Manager.
SANTA CLARA, CA – Rebus Biosystems Inc, (“Rebus Bio”, “Rebus”), an Illumina Ventures-backed life science technology company creating revolutionary tools to advance spatial omics research, today announced Danilo Tait as the newest member of its leadership team. Tait, who holds a PhD in molecular biology from the Max-Planck Institute for Molecular Genetics and an MBA from SMC University in […]
Quantification of gene expression changes in mouse disease models using a high-throughput spatial omics platform
Batch effects due to technical variability are a major problem in single cell transcriptomics. Spatial methods are no exception – their low throughput requires high numbers of technical replicates, reducing the statistical power needed to quantify differential gene expression across experimental conditions. To overcome this problem, we took advantage of the large imaging area of […]
We demonstrate the ability of the Rebus Esper platform combined with the Esper High Fidelity assay to identify all major cell types of the mouse brain and faithfully reconstruct the known tissue architecture using spatial data from only 17 cell type-specific genes and 7 reference genes.
The Rebus Esper is an integrated, automated spatial omics platform that delivers quantitative single-molecule, single-cell data with subcellular resolution.
The Esper High Fidelity assay, based on gold-standard smFISH, enables multiplex analysis of gene expression with no compromise in sensitivity or specificity.
The Rebus Esper is an integrated, automated platform that brings together advancements in imaging, fluidics, chemistry and bioinformatics to deliver spatial omics data without compromise.
The Rebus Esper makes no compromises when it comes to ease of use. Preparing a run follows a simple protocol that requires less than one hour of hands-on time. Using the instrument is as easy as loading the reagents and flow cell, starting the run, and coming back at the end to collect your processed […]
Aparna Bhaduri, PhD, Assistant Professor of Biological Chemistry at the David Geffen School of Medicine at UCLA talks about her neuroscience research, including a deep dive into the use of Rebus Esper-enabled spatial omics data to understand the diversity of cell types across regions of the developing human cortex.
The Rebus Esper enables analysis of cells in their native tissue contexts at high throughput and provides rapid analysis of biological molecules directly in and across large tissue sections with pristine subcellular detail. Rebus Biosystems Director of Marketing Erin Davis explains more in this installment of the Spatial Omics webinar series.
The Rebus Esper brings together advancements in imaging, fluidics, chemistry and bioinformatics to deliver spatial omics data without compromise. Rebus Biosystems Senior Product Manager Brett Cook explains more about how this revolutionary system brings quantitative single-molecule, single-cell spatial data with subcellular resolution to your research.
In conventional light microscopy, the physics of lenses impose relations between resolution and other optical parameters of the system. For example, the numerical aperture of a lens cannot be increased to improve resolution without either increasing the size of the lens or decreasing the working distance. The technique presented here, Synthetic Aperture Optics (SAO), removes […]
DIGIT is a Conserved Long Noncoding RNA that Regulates GSC Expression to Control Definitive Endoderm Differentiation of Embryonic Stem Cells
Long noncoding RNAs (lncRNAs) exhibit diverse functions, including regulation of development. Here the authors combined genome-wide mapping of SMAD3 occupancy with expression analysis to identify lncRNAs induced by activin signaling during endoderm differentiation of human embryonic stem cells (hESCs). They found that DIGIT is divergent to Goosecoid (GSC) and expressed during endoderm differentiation. Using single […]
Cooperation between DNA, RNA and protein regulates gene expression and controls differentiation through interactions connecting regions of nucleic acids and protein domains and through the assembly of biomolecular condensates. In this study, the authors used StellarVision, a Synthetic Aperture Optics-based imaging system, to measure interactions between DIGIT, a long non-oding RNA, and BRD3, a protein […]
Mutations in the Promoter of the Telomerase Gene TERT Contribute to Tumorigenesis by a Two-step Mechanism
Mutations in the promoter region of the gene coding for the reverse transcriptase component of telomerase (TERT) are found at high frequency in various human tumor types. These TERT promoter mutations (TPMs) have been detected both in early- and late-stage tumors, consistent with the possibility that they play a role early in tumorigenesis. SAO-based imaging […]
Validating scRNAseq Data from Human Fetal Brain Using Automated, Highly Multiplexed in situ mRNA Detection with Single-cell and Single-transcript Resolution
Single-cell RNA sequencing (scRNA-seq) has fundamentally expanded our understanding of tissue composition and heterogeneity. While this methodology enables unbiased and comprehensive identification of distinct cell types based on their transcriptomic profiles, it does not provide positional context in the tissue architecture, which is critical for understanding the interactions between cells and their native tissue microenvironment. […]